Dissemination of 6S RNA among Bacteria
نویسندگان
چکیده
منابع مشابه
Legionella pneumophila 6S RNA optimizes intracellular multiplication.
Legionella pneumophila is a Gram-negative opportunistic human pathogen that infects and multiplies in a broad range of phagocytic protozoan and mammalian phagocytes. Based on the observation that small regulatory RNAs (sRNAs) play an important role in controlling virulence-related genes in several pathogenic bacteria, we attempted to identify sRNAs expressed by L. pneumophila. We used computati...
متن کامل6S RNA Regulates E. coli RNA Polymerase Activity
The E. coli 6S RNA was discovered more than three decades ago, yet its function has remained elusive. Here, we demonstrate that 6S RNA associates with RNA polymerase in a highly specific and efficient manner. UV crosslinking experiments revealed that 6S RNA directly contacts the sigma70 and beta/beta' subunits of RNA polymerase. 6S RNA accumulates as cells reach the stationary phase of growth a...
متن کاملBinding and release of the 6S transcriptional control RNA.
6S RNA is an important noncoding RNA that regulates eubacterial transcription. In Escherichia coli this RNA binds to the sigma(70) RNA polymerase holoenzyme and is released by the synthesis of a short product RNA. In order to determine how binding and release are controlled by the 6S RNA sequence, we used in vitro selection to screen a high diversity library containing approximately 4 x 10(12) ...
متن کامل6S RNA function enhances long-term cell survival.
6S RNA was identified in Escherichia coli >30 years ago, but the physiological role of this RNA has remained elusive. Here, we demonstrate that 6S RNA-deficient cells are at a disadvantage for survival in stationary phase, a time when 6S RNA regulates transcription. Growth defects were most apparent as a decrease in the competitive fitness of cells lacking 6S RNA. To decipher the molecular mech...
متن کاملWide dissemination of a carbapenemase plasmid among gram-negative bacteria: implications of the variable phenotype.
Broad-spectrum carbapenem antibiotics are the last bastion of empirical therapy for resistant gram-negative bacterial infections, and outbreaks of carbapenem-resistant bacterial infection may be associated with greatly increased mortality in the critically ill (4). Carbapenems (e.g., imipenem, meropenem, ertapenem) are increasingly threatened by dissemination of metallo-lactamases (M Ls), the m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: RNA Biology
سال: 2014
ISSN: 1547-6286,1555-8584
DOI: 10.4161/rna.29894